| Field | Specification |
|---|---|
| Target | |
| Alternative names | OTU domain-containing protein 5; Deubiquitinating enzyme A; DUBA; OTUD5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human OTUD5 recombinant protein (Position: R168-D555). Human OTUD5 shares 98.2% and 98.5% amino acid (aa) sequence identity with mouse and rat OTUD5, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects OTU domain-containing protein 5 (gene OTUD5) in human and rat samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 60.6 kDa, and the supplier reports an observed band at about 75 kDa.
OTUD5 (also called DUBA) is an OTU-family deubiquitinase that restrains innate immune responses. It removes ubiquitin chains from TRAF3 to suppress type I interferon production, and it also stabilizes chromatin regulators during neuroectodermal development.
| Target | OTU domain-containing protein 5 (gene OTUD5; UniProt Q96G74, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 60.6 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human Jurkat cells, human K562 cells, rat liver tissue, rat C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Recombinant human OTUD5 fragment (Arg168–Asp555), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98.2% and 98.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places OTU domain-containing protein 5 in the nucleus. Tissue expression noted by UniProt (human): Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
