| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin thioesterase otulin; Deubiquitinating enzyme otulin; OTU domain-containing deubiquitinase with linear linkage specificity; Ubiquitin thioesterase Gumby; OTULIN; FAM105B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human OTULIN recombinant protein (Position: A49-L352). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ubiquitin thioesterase otulin in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 40.3 kDa, and the supplier reports an observed band at about 43–45 kDa.
OTULIN (also called FAM105B or Gumby) is a deubiquitinase that cuts only linear, Met1-linked polyubiquitin. It mainly keeps the LUBAC ligase complex in check by removing its self-attached linear chains, which limits NF-κB-driven inflammation; loss of OTULIN causes a severe autoinflammatory syndrome, and the enzyme also contributes to Wnt signaling and angiogenesis.
| Target | Ubiquitin thioesterase otulin (gene OTULIN; UniProt Q96BN8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 40.3 kDa |
| Observed MW | 43–45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human THP-1 cells, human K562 cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:100, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human appendicitis tissue, human bladder cancer tissue, human tonsil tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): SIHA cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human OTULIN fragment (Ala49–Leu352), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin thioesterase otulin in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kaya Sercan et al. (2023) In an experimental myocardial infarction model, L-arginine pre-intervention may exert cardioprotective effects by regulating OTULIN levels and mitochondrial dynamics. Cell Stress & Chaperones. 10.1007/s12192-023-01373-6
Tuba Yalçın et al. (2024) Therapeutic Potential of the Linalool against Cadmium-Induced Testicular Tissue Damage. Journal of Trace Elements in Medicine and Biology. 10.1016/j.jtemb.2024.127455
Tuba Yalcın et al. (2023) Resveratrol may dose-dependently modulate nephrin and OTULIN levels in a doxorubicin-induced nephrotoxicity model. Toxicology Mechanisms and Methods. 10.1080/15376516.2023.2268717
Kaya Sercan et al. (2026) Dynamic changes in OTULIN and progranulin levels in experimental myocardial infarction and cardiac remodeling. Histochemistry and Cell Biology. 10.1007/s00418-026-02506-5
Yalçın Tuba et al. (2024) The Effect of Thymoquinone on the TNF-α/OTULIN/NF-κB Axis Against Cisplatin-İnduced Testicular Tissue Damage. Reproductive Sciences. 10.1007/s43032-024-01567-y
Yalçın Tuba et al. (2026) Effect of Hydroxytyrosol on OTULIN Levels in Testıcular Tıssue in Experımental Dıabetes Model Induced wıth Streptozotocin. Reproductive Sciences. 10.1007/s43032-026-02085-9
