| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein OTX1;Orthodenticle homolog 1;OTX1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Otx1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BDH-15 is a rabbit monoclonal antibody that recognizes homeobox protein OTX1 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 37.3 kDa, and the supplier reports an observed band at about 37 kDa.
OTX1 is a homeodomain transcription factor related to Drosophila orthodenticle. It recognizes bicoid-type DNA sites and helps pattern the developing forebrain, midbrain and sense organs, so it is studied in brain development and cortical neuron specification.
| Target | Homeobox protein OTX1 (gene OTX1; UniProt P32242, human) |
|---|---|
| Clone | BDH-15 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 37.3 kDa |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human otx1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places homeobox protein OTX1 in the nucleus. Tissue expression noted by UniProt (human): Expressed in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jing Li et al. (2020) The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p. Oncology Research. 10.3727/096504019X15619783964700
