| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein OTX2; Orthodenticle homolog 2; OTX2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Otx2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21O72 is a rabbit monoclonal antibody that recognizes homeobox protein OTX2 in human samples and is validated for Western blot. The predicted molecular weight is 31.6 kDa, and the supplier reports an observed band at about 32 kDa.
OTX2 is a bicoid-class homeodomain transcription factor needed to form the anterior head and brain. It controls forebrain and midbrain patterning and eye development, including retinal pigment epithelium and photoreceptor differentiation, and is a common marker in neurodevelopment and retinal research.
| Target | Homeobox protein OTX2 (gene OTX2; UniProt P32243, human) |
|---|---|
| Clone | 21O72 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.6 kDa |
| Observed MW | 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Y79 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human otx2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places homeobox protein OTX2 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
