| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase OSR1; Oxidative stress-responsive 1 protein; OXSR1; KIAA1101; OSR1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human OXSR1 recombinant protein (Position: K351-S527). Human OXSR1 shares 89.8% and 85.4% amino acid (aa) sequence identity with mouse and rat OXSR1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes serine/threonine-protein kinase OSR1 (gene OXSR1) in human, mouse and rat samples and is validated for Western blot, flow cytometry, immunoprecipitation and ELISA. The predicted molecular weight is 58.0 kDa, and the supplier reports an observed band at about 58 kDa.
OSR1 (OXSR1) is a serine/threonine kinase acting downstream of WNK kinases in the WNK–SPAK/OSR1 cascade. Once activated, it phosphorylates cation-chloride cotransporters such as NKCC1, NKCC2 and NCC, and inhibits KCC family members, which controls cell volume, renal salt reabsorption and blood pressure.
| Target | Serine/threonine-protein kinase OSR1 (gene OXSR1; UniProt O95747, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 58.0 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human Jurkat cells, human U251 cells, rat liver tissue, rat testis tissue, mouse liver tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed result (IP): U251 cells.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human OXSR1 fragment (Lys351–Ser527), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 89.8% and 85.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase OSR1 in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed in all tissue examined. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
