| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oxytocin receptor; OT-R; OXTR |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Oxytocin Receptor |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22O37 is a rabbit monoclonal antibody that recognizes oxytocin receptor (gene OXTR) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 42.8 kDa, and the supplier reports an observed band at about 43 kDa.
The oxytocin receptor (OXTR) is a G protein-coupled receptor that mainly signals through Gq, phospholipase C and Ca2+ release. It mediates uterine contraction during labor and milk ejection during lactation, and in the brain it shapes maternal care, social recognition and bonding.
| Target | Oxytocin receptor (gene OXTR; UniProt P30559, human) |
|---|---|
| Clone | 22O37 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.8 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human Jurkat cells, human PC-3 cells, human A549 cells, human Caco-2 cells, rat PC-12 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): rat ovary tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human oxytocin Receptor.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places oxytocin receptor in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hanniely Thais Menezes Santos et al. (2026) The hemoglobin-derived peptide LVV-H7 attenuates select behavioral alterations in male spontaneously hypertensive rats. Neuropeptides. 10.1016/j.npep.2026.102636
