| Field | Specification |
|---|---|
| Target | |
| Alternative names | Multidrug resistance protein 1;3.6.3.44;ATP-binding cassette sub-family B member 1;P-glycoprotein 1;CD243;ABCB1;MDR1, PGY1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human P Glycoprotein |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FDB-1 is a rabbit monoclonal antibody that recognizes ATP-dependent translocase ABCB1 in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 141.5 kDa, and the supplier reports an observed band at about 180 kDa.
P-glycoprotein (ABCB1, MDR1) is an ATP-powered efflux pump in the plasma membrane that expels a wide range of drugs and moves certain phospholipids across the membrane. High ABCB1 levels are a classic cause of multidrug resistance in cancer cells, and it also shapes drug absorption and the blood-brain barrier.
| Target | ATP-dependent translocase ABCB1 (gene ABCB1; UniProt P08183, human) |
|---|---|
| Clone | FDB-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 141.5 kDa |
| Observed MW | 180 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with confirmed staining (IHC): rat heart tissue, human cardiac muscle tissue, mouse skin tissue, rat spleen tissue, human adrenal gland tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human P Glycoprotein.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-dependent translocase ABCB1 in the cell membrane, apical cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaoying Wang et al. (2022) Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2022.122138
Fei Wang et al. (2020) HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway. European Journal of Pharmacology. 10.1016/j.ejphar.2020.173189
Feifei Wang et al. (2019) Hydroxychloroquine reverses the drug resistance of leukemic K562/ADM cells by inhibiting autophagy. Molecular Medicine Reports. 10.3892/mmr.2019.10621
