| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cyclin-dependent kinase inhibitor 2A ;Cyclin-dependent kinase 4 inhibitor A;CDK4I;p16-INK4a;p16;p16-INK4;Cdkn2a ;P16ink4a ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant human p16 protein. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) is an antibody targeting CDKN2A. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: DCS-50; isotype: Mouse IgG1; reactivity: Human; observed MW: 200 kDa; calculated MW: 17366 MW.
Boster Bio Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) catalog # MA1074. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CDKN2A — Cyclin-dependent kinase inhibitor 2A
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: DCS-50; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 200 kDa; Calculated: 17366 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity). .
Scientific background (datasheet): p16 (INK4A), also known as cyclin-dependent kinase inhibitor 2A (CDKN2A), or multiple tumor suppressor 1 (MTS1). The p16 gene (CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma.
Cellular localization (datasheet): Cytoplasm . Nucleus .
Tissue details (datasheet): Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. .
Sequence similarities (datasheet): Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cancer Susceptibility,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,Epigenetics and Nuclear Signaling,Oncoproteins/Suppressors,p53 Pathway,Transcription,Tumor Suppressors.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
