| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase inhibitor 2A ;Cyclin-dependent kinase 4 inhibitor A;CDK4I;p16-INK4a;p16;p16-INK4;Cdkn2a ;P16ink4a ; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant human p16 protein. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DCS-50 is a mouse monoclonal antibody that recognizes cyclin-dependent kinase inhibitor 2A (gene Cdkn2a) in human samples and is validated for Western blot, IHC and ICC/IF.
CDKN2A encodes p16INK4a, a cyclin-dependent kinase inhibitor that binds CDK4 and CDK6 and prevents them from partnering with D-type cyclins to phosphorylate RB1. The locus also encodes the distinct protein p14ARF; p16 is a widely used marker of cellular senescence and one of the most frequently inactivated tumor suppressors in human cancer.
| Target | Cyclin-dependent kinase inhibitor 2A (gene Cdkn2a; UniProt Q9R0Z3, rat) |
|---|---|
| Clone | DCS-50 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 5 µg/mL (Human) |
|---|---|
| Western blot | Not specified by the supplier |
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human p16 protein.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cyclin-dependent kinase inhibitor 2A in the cytoplasm and nucleus. Tissue expression noted by UniProt (rat): Expressed in spleen, liver and lung.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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