| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase 4 inhibitor D; p19-INK4d; CDKN2D |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human p19 INK4d/CDKN2D recombinant protein (Position: E31-H132). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cyclin-dependent kinase 4 inhibitor D (gene CDKN2D) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 17.7 kDa, and the supplier reports an observed band at about 18 kDa.
p19INK4d (CDKN2D) is an INK4 family cyclin-dependent kinase inhibitor that binds CDK4 and CDK6 and blocks their activity. By restraining G1 progression it helps control cell proliferation, and it is studied in cell cycle regulation and differentiation.
| Target | Cyclin-dependent kinase 4 inhibitor D (gene CDKN2D; UniProt P55273, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 17.7 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MOLT4 cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human p19 INK4d/CDKN2D fragment (Glu31–His132), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cyclin-dependent kinase 4 inhibitor D in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ruosha Lai et al. (2015) Role of p19ink4d in the pathogenesis of hearing loss. International Journal of Clinical and Experimental Pathology. PMID: 26722409
