| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase inhibitor 1B;Cyclin-dependent kinase inhibitor p27;p27Kip1;CDKN1B;KIP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cyclin-dependent kinase inhibitor 1B (gene CDKN1B) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 27 kDa.
p27 (CDKN1B, p27Kip1) is a cyclin-dependent kinase inhibitor that holds cells in G1 by blocking cyclin E-CDK2 and cyclin A-CDK2 activity. Its levels are tightly controlled by phosphorylation and degradation, and it is studied as a regulator of proliferation, quiescence and tumor suppression.
| Target | Cyclin-dependent kinase inhibitor 1B (gene CDKN1B; UniProt P46527, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 22.1 kDa |
| Observed MW | 27 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human P27 KIP 1 fragment (Ser10–Thr198), expressed in E. coli. Sequence identity with the mouse ortholog: 87%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cyclin-dependent kinase inhibitor 1B in the nucleus, cytoplasm and endosome. Tissue expression noted by UniProt (human): Expressed in kidney (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fuqiang Xu et al. (2013) The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells. Cancer Letters. 10.1016/j.canlet.2013.01.029
Ben Hui Li et al. (2011) Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo. Cellular Signalling. 10.1016/j.cellsig.2011.11.005
Zhikun Liu et al. (2018) Metallothionein 1 family profiling identifies MT1X as a tumor suppressor involved in the progression and metastastatic capacity of hepatocellular carcinoma. Molecular Carcinogenesis. 10.1002/mc.22846
Y. Dai et al. (2012) MicroRNA-155 inhibits proliferation and migration of human extravillous trophoblast derived HTR-8/SVneo cells via down-regulating cyclin D1. Placenta. 10.1016/j.placenta.2012.07.012
Zhu Zhichuan et al. (2013) ZFX regulates glioma cell proliferation and survival in vitro and in vivo. Journal of Neuro-Oncology. 10.1007/s11060-012-1032-z
Wang Tianzhen et al. (2011) Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells. Virology Journal. 10.1186/1743-422X-8-231
Kefu Cai et al. (2009) Dynamic changes of cell cycle elements in the ischemic brain after bone marrow stromal cells transplantation in rats. Neuroscience Letters. 10.1016/j.neulet.2009.09.052
Deng-Ke Yin et al. (2007) Chemically Modified Heparin Inhibits Mesangial Cell Proliferation Induced by High Glucose through Interfering with the Cell Cycle. Biological & Pharmaceutical Bulletin. 10.1248/bpb.30.2274
Chen Xianxiang et al. (2010) Effects of Ad-p27mt gene transfer on the expression of Bax, Bcl-2, VEGF and MMP-9 in the transplanted liver tumors in nude mice. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-010-0551-0
