| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | P2X purinoceptor 4;P2X4;ATP receptor;Purinergic receptor;P2RX4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human P2RX4 recombinant protein (Position: N262-Q388). Human P2RX4 shares 91% and 90% amino acid (aa) sequence identity with mouse and rat P2RX4, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of P2RX4 (P2X purinoceptor 4) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-P2RX4 Antibody Picoband® catalog # PB9304. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human P2RX4 recombinant protein (Position: N262-Q388). Human P2RX4 shares 91% and 90% amino acid (aa) sequence identity with mouse and rat P2RX4, respectively. (reported region: N262-Q388).
- Molecular weight context: reported MW: 72 kDa; calculated MW: 43369 MW
- Reactivity: Human,Monkey,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
P2X purinoceptor 4; P2X purinoceptor 4. Purinoceptor P2X4, also called P2X4R is a protein that in humans is encoded by the P2RX4 gene. This gene belongs to the family of purinoceptors for ATP. P2RX4 was mapped to 12q24.32 by fluorescence in situ hybridization. P2RX4 is a receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. P2X4 receptor in hyperactive microglia is necessary for tactile allodynia after nerve injury and is sufficient to produce tactile allodynia in normal animals. P2X4 recetors have beeb implicated in the regulation of cardiac function, ATP-mediated cell death, synaptic strengthening, and activating of the inflammasome in response to injury. Functional note: Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. . Reported localization: Membrane ; Multi-pass membrane protein .
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how P2RX4 (P2X purinoceptor 4) relates to this theme using model systems and orthogonal readouts.
- Ligand-Gated Ion Channels: Researchers commonly examine how P2RX4 (P2X purinoceptor 4) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how P2RX4 (P2X purinoceptor 4) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative P2RX4 (P2X purinoceptor 4) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the P2X receptor family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
