| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | P2X purinoceptor 2;P2X2;ATP receptor;Purinergic receptor;P2RX2;P2X2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human P2X2 recombinant protein (Position: D139-L471). Human P2X2 shares 84% amino acid (aa) sequence identity with both mouse and rat P2X2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of P2RX2 (P2X purinoceptor 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-P2X2/P2RX2 Antibody Picoband® catalog # PB9303. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human P2X2 recombinant protein (Position: D139-L471). Human P2X2 shares 84% amino acid (aa) sequence identity with both mouse and rat P2X2. (reported region: D139-L471).
- Molecular weight context: reported MW: 60 kDa; calculated MW: 51754 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
P2X purinoceptor 2; P2X purinoceptor 2. The P2RX2 gene encodes the P2X2 receptor, which assembles as a trimer to form a ligand-gated ion channel gated by extracellular ATP. P2X2 receptors mediate a variety of cellular responses, including excitatory postsynaptic responses in sensory neurons. The product of this gene belongs to the family of purinoceptors for ATP. P2RX2 is mapped to 12q24.33. It has been found that ATP-activated P2RX2 influenced OHC electromotility, a stimulus-induced change in hair cell length that functions as an amplifier to determine hearing sensitivity and frequency selectivity. What’s more, P2RX2 channels were necessary for development of the temporary threshold shift. Functional note: Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K (+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle. . Reported localization: Cell membrane ; Multi-pass membrane protein . Localizes to the apical membranes of hair cells in the organ of Corti.
Research relevance and current trends
- Cancer: Researchers commonly examine how P2RX2 (P2X purinoceptor 2) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how P2RX2 (P2X purinoceptor 2) relates to this theme using model systems and orthogonal readouts.
- Ligand-Gated Ion Channels: Researchers commonly examine how P2RX2 (P2X purinoceptor 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative P2RX2 (P2X purinoceptor 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of P2RX2 (P2X purinoceptor 2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the P2X receptor family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
