| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Heat shock factor protein 2; HSF 2; Heat shock transcription factor 2; HSTF 2; HSF2; HSTF2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human P2X4/P2RX4 recombinant protein (Position: Q111-E249). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-P2X4/P2RX4 Antibody Picoband® is an antibody for P2RX4 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: P2RX4 (heat shock transcription factor 2); UniProt: Q99571
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 70 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-P2X4/P2RX4 Antibody Picoband® catalog # A04715-2.
Biological background
Biological context: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Expression and localization notes: cellular localization: Cytoplasm..
Common research applications
- Western blotting (WB): Compare P2RX4 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of P2RX4 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify P2RX4-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Purinoceptor P2X4, also called P2X4R is a protein that in humans is encoded by the P2RX4 gene. This gene belongs to the family of purinoceptors for ATP. P2RX4 was mapped to 12q24.32 by fluorescence in situ hybridization. P2RX4 is a receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. P2X4 receptor in hyperactive microglia is necessary for tactile allodynia after nerve injury and is sufficient to produce tactile allodynia in normal animals. P2X4 recetors have beeb implicated in the regulation of cardiac function, ATP-mediated cell death, synaptic strengthening, and activating of the inflammasome in response to injury.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm.
- Research category: Epigenetics and Nuclear Signaling,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
