| Field | Specification |
|---|---|
| Target | |
| Alternative names | P2Y purinoceptor 12; P2Y12; ADP-glucose receptor; ADPG-R; P2T(AC); P2Y(AC); P2Y(cyc); P2Y12 platelet ADP receptor; P2Y(ADP); SP1999; P2RY12; HORK3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human P2Y12 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28P17 is a rabbit monoclonal antibody that recognizes P2Y purinoceptor 12 (gene P2RY12) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 39.4 kDa, and the supplier reports an observed band at about 50 kDa.
P2Y12 is a Gi-coupled receptor for ADP that lowers cAMP when activated. On platelets it is essential for sustained aggregation and clot formation and is the target of antiplatelet drugs such as clopidogrel; in the brain it is a well-known marker of homeostatic microglia.
| Target | P2Y purinoceptor 12 (gene P2RY12; UniProt Q9H244, human) |
|---|---|
| Clone | 28P17 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.4 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human Jurkat cells, human SH-SY5Y cells, human U-87 MG cells, human U251 cells, rat brain tissue, rat C6 cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human P2Y12.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places P2Y purinoceptor 12 in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed in the platelets, lower levels in the brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuan Wang et al. (2026) Microglial P2RY12 mediates migration to and protection of cerebral microvasculature after ischemia–reperfusion via Caveolin-1. Experimental Neurology. 10.1016/j.expneurol.2026.115662
