| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; p57Kip2; CDKN1C; KIP2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human p57 Kip2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17C19 is a rabbit monoclonal antibody that recognizes cyclin-dependent kinase inhibitor 1C (gene CDKN1C) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 32.2 kDa, and the supplier reports an observed band at about 45–54 kDa.
p57 (CDKN1C, p57Kip2) is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors that binds tightly to G1 cyclin-CDK complexes to restrain proliferation. It is an imprinted gene expressed mainly from the maternal allele, and it is studied in embryonic growth control and imprinting disorders such as Beckwith-Wiedemann syndrome.
| Target | Cyclin-dependent kinase inhibitor 1C (gene CDKN1C; UniProt P49918, human) |
|---|---|
| Clone | 17C19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 32.2 kDa |
| Observed MW | 45–54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human thyroid cancer tissue, human ovarian cancer tissue, rat kidney tissue, human tongue cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human p57 Kip2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-dependent kinase inhibitor 1C in the nucleus. Tissue expression noted by UniProt (human): Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xingsheng Hu et al. (2024) The expression and role of SUZ12 in lung adenocarcinoma. Cancer Medicine. 10.1002/cam4.70190
