| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sequestosome-1;EBI3-associated protein of 60 kDa;EBIAP;p60;Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa;Ubiquitin-binding protein p62;SQSTM1;ORCA, OSIL; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human p62/SQSTM1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EBB-19 is a rabbit monoclonal antibody that recognizes sequestosome-1 (gene SQSTM1) in human, mouse and rat samples and is validated for flow cytometry, immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 47.7 kDa, and the supplier reports an observed band at about 62 kDa.
Sequestosome-1 (SQSTM1, also known as p62) is an autophagy receptor that binds polyubiquitinated proteins and LC3 on autophagosomes, delivering protein aggregates for degradation. It also acts as a signaling hub for pathways such as KEAP1-NRF2, and because it is itself degraded by autophagy, p62 is widely used as a marker of autophagic flux.
| Target | Sequestosome-1 (gene SQSTM1; UniProt Q13501, human) |
|---|---|
| Clone | EBB-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.7 kDa |
| Observed MW | 62 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human A549 cells, human RT4 cells, human HeLa cells, rat brain tissue, rat PC-12 cells, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:5000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human spleen tissue, human cervix squamous cells carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human p62/SQSTM1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sequestosome-1 in the cytoplasmic vesicle, preautophagosomal structure, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaoying Qian et al. (2026) Ionizing radiation promotes lung injury by inducing ferroptosis-driven senescence in epithelial cells via NCOA4-mediated ferritinophagy. Redox Biology. 10.1016/j.redox.2026.104091
Zhang Haihang et al. (2024) Mitoxantrone ameliorates ineffective erythropoiesis in a β-thalassemia intermedia mouse model. Blood Advances. 10.1182/bloodadvances.2024012679
Zhirui Zheng et al. (2025) Salidroside Derivative SHPL-49 Exerts Anti-Neuroinflammatory Effects by Modulating Excessive Autophagy in Microglia. Cells. 10.3390/cells14060425
Zhou Cefan et al. (2019) Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction. Cell Communication and Signaling. 10.1186/s12964-019-0422-7
Yingbin Chai et al. (2025) Polyphyllin I Inhibits the Metastasis of Cervical Cancer Through the Regulation of the β-Catenin Signaling Pathway. International Journal of Molecular Sciences. 10.3390/ijms26104630
Huang Yuan et al. (2020) Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy. Frontiers in Oncology. 10.3389/fonc.2020.573127
Ji-Xian Song et al. (2025) Hydrogen alleviates cardiac ferroptosis damage under chronic intermittent hypoxia conditions by targeting Nrf2. International Immunopharmacology. 10.1016/j.intimp.2025.115974
Yarong Sun et al. (2025) Fu's Subcutaneous Needling Therapy Significantly Enhances Pain Relief in Sciatica Rats by Promoting Mitochondrial Autophagy. Biomedical Journal. 10.1016/j.bj.2025.100927
Zhitao Li et al. (2025) GLDC attenuates liver ischemia-reperfusion injury by inhibiting macrophage recruitment and activation via PTBP1/P2RY6. Cellular Signalling. 10.1016/j.cellsig.2025.111976
Wencong Ding et al. (2023) Lower dose of metformin combined with artesunate induced autophagy-dependent apoptosis of glioblastoma by activating ROS-AMPK-mTOR axis. Experimental Cell Research. 10.1016/j.yexcr.2023.113691
