| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ribosomal protein S6 kinase beta-2; S6K-beta-2; S6K2; 70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta; S6K-beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70-beta; RPS6KB2; STK14B |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human P70 S6 Kinase beta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19R00 is a rabbit monoclonal antibody that recognizes ribosomal protein S6 kinase beta-2 (gene RPS6KB2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 53.5 kDa, and the supplier reports an observed band at about 53 kDa.
S6K2 (RPS6KB2) is a p70 ribosomal S6 kinase closely related to S6K1. It is activated downstream of mTOR signaling and phosphorylates ribosomal protein S6, contributing to growth-factor and nutrient control of cell growth, and it is more concentrated in the nucleus than S6K1.
| Target | Ribosomal protein S6 kinase beta-2 (gene RPS6KB2; UniProt Q9UBS0, human) |
|---|---|
| Clone | 19R00 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.5 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human P70 S6 Kinase beta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ribosomal protein S6 kinase beta-2 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Zhaoyu et al. (2025) USF1-induced RPS6KB2 activation influences aggressive phenotype in B-cell non-Hodgkin lymphoma. Human Cell. 10.1007/s13577-025-01284-x
