| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor protein p73;p53-like transcription factor;p53-related protein;TP73;P73; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human p73 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EHI-20 is a rabbit monoclonal antibody that recognizes tumor protein p73 (gene TP73) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 69.6 kDa, and the supplier reports an observed band at about 73 kDa.
p73 (TP73) is a transcription factor of the p53 family. Isoforms with a transactivation domain can promote apoptosis after DNA damage, whereas ΔN isoforms lacking this domain oppose p53 and full-length p73. p73 is also needed for the development of multiciliated cells and parts of the nervous system, and it is studied in cancer and neurodevelopment.
| Target | Tumor protein p73 (gene TP73; UniProt O15350, human) |
|---|---|
| Clone | EHI-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.6 kDa |
| Observed MW | 73 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human THP-1 cells, human U2OS cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human skin cancer tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue, mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human p73.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tumor protein p73 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in striatal neurons of patients with Huntington disease (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xu Deng et al. (2019) Cinobufagin Promotes Cell Cycle Arrest and Apoptosis to Block Human Esophageal Squamous Cell Carcinoma Cells Growth via the p73 Signalling Pathway. Biological & Pharmaceutical Bulletin. 10.1248/bpb.b19-00174
