| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor protein p73;p53-like transcription factor;p53-related protein;TP73;P73; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human p73 recombinant protein (Position: M1-E198). Human p73 shares 96% amino acid (aa) sequence identity with mouse p73. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tumor protein p73 (gene TP73) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 69.6 kDa, and the supplier reports an observed band at about 73 kDa.
p73 (TP73) is a transcription factor of the p53 family. Isoforms with a transactivation domain can promote apoptosis after DNA damage, whereas ΔN isoforms lacking this domain oppose p53 and full-length p73. p73 is also needed for the development of multiciliated cells and parts of the nervous system, and it is studied in cancer and neurodevelopment.
| Target | Tumor protein p73 (gene TP73; UniProt O15350, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 69.6 kDa |
| Observed MW | 73 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells, MCF-7 cells, COLO320 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human intestinal cancer tissue, rat brain tissue, mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant human p73 fragment (Met1–Glu198), expressed in E. coli. Sequence identity with the mouse ortholog: 96%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tumor protein p73 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in striatal neurons of patients with Huntington disease (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
