| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein kinase C and casein kinase substrate in neurons protein 1; Syndapin-1; PACSIN1; KIAA1379 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PACSIN1 recombinant protein (Position: Q76-E359). Human PACSIN1 shares 95.8% and 96.5% amino acid (aa) sequence identity with mouse and rat PACSIN1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes protein kinase C and casein kinase substrate in neurons protein 1 (gene PACSIN1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 51.0 kDa, and the supplier reports an observed band at about 51 kDa.
PACSIN1 (syndapin I) is a neuron-enriched F-BAR protein that binds and bends membranes. It recruits dynamin and actin regulators such as N-WASP and cobl, supporting synaptic vesicle endocytosis and the growth and branching of neurites.
| Target | Protein kinase C and casein kinase substrate in neurons protein 1 (gene PACSIN1; UniProt Q9BY11, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 51.0 kDa |
| Observed MW | 51 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human glioblastoma tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): Jurkat cells, K562 cells.
Immunogen
Recombinant human PACSIN1 fragment (Gln76–Glu359), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95.8% and 96.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein kinase C and casein kinase substrate in neurons protein 1 in the cytoplasm, cell projection, synapse and membrane. Tissue expression noted by UniProt (human): Highly expressed in brain and, at much lower levels, in heart and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
