| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-PACSIN3 Antibody is an antibody targeting Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII (also reported as Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII).
- Immunogen/epitope region: Intracellular.
- Homology note: Rat, human - identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Pacsin 3 (also known as Syndapin 3) is a member of the highly conserved Pacsin protein sub-family which constitutes a branch of the larger F-BAR domain protein family.Pacsin 3, as well as other members of its family, is a cytoplasmic protein involved in receptor-mediated endocytosis, synaptic vesicle trafficking and biogenesis of different cellular organelles and is mostly, but not only, expressed in lung and muscle tissues. Pacsin 3 contains an N-terminal F-BAR domain and a C-terminal mono-Src homology 3 domain1.The C-terminal SH3 domain of Pacsin 3 interacts with the N-terminal proline-rich domain of TRPV4 at residues 132-144 and enhances the cell surface expression of TRPV4, probably through the inhibition of the endocytotic process2. The SH3 domain is also responsible for interactions with the protein dynamin and with N-WASP, a potent activator of the Arp2/3 complex F-actin-nucleation machine3.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-IP015.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-IP015; Negative control: BLP-IP015.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
