| Field | Specification |
|---|---|
| Target | |
| Alternative names | Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1; Serpine1; Pai1; Planh1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat PAI1 recombinant protein (Position: S24-D240). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets plasminogen activator inhibitor 1 (gene Serpine1) in mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 45.0 kDa, and the supplier reports an observed band at about 45–50 kDa.
Plasminogen activator inhibitor 1 (PAI-1, SERPINE1) is the main inhibitor of tissue-type and urokinase-type plasminogen activators and so acts as a brake on fibrinolysis. It also influences cell adhesion and migration and is strongly induced by TGF-β and other stimuli, making it a common target in studies of thrombosis, fibrosis, metabolism and cancer.
| Target | Plasminogen activator inhibitor 1 (gene Serpine1; UniProt P20961, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 45.0 kDa |
| Observed MW | 45–50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat small intestine tissue, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant rat PAI1 fragment (Ser24–Asp240), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes plasminogen activator inhibitor 1 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Rumei Luan et al. (2023) Zinc deficiency increases lung inflammation and fibrosis in obese mice by promoting oxidative stress. Biochimica et Biophysica ACTA-General Subjects. 10.1016/j.bbagen.2023.130518
