| Field | Specification |
|---|---|
| Target | |
| Alternative names | Palladin; SIH002; Sarcoma antigen NY-SAR-77; PALLD; KIAA0992 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Palladin/PALLD recombinant protein (Position: H89-L1383). Human PALLD shares 85.5% amino acid (aa) sequence identity with mouse PALLD. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects palladin (gene PALLD) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 150.6 kDa, and the supplier reports an observed band at about 95 kDa.
Palladin (PALLD) is an actin-associated scaffold protein found in stress fibers, focal adhesions and lamellipodia. It binds several actin-binding partners, including α-actinin, and helps organize actin bundles that control cell shape, adhesion and migration.
| Target | Palladin (gene PALLD; UniProt Q8WX93, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 150.6 kDa |
| Observed MW | 95 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Palladin/PALLD fragment (His89–Leu1383), expressed in E. coli. Sequence identity with the mouse ortholog: 85.5%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places palladin in the cytoplasm, cell junction and cell projection. Tissue expression noted by UniProt (human): Detected in both muscle and non-muscle tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
