Anti-PAR1/Thrombin Receptor/F2R Antibody Picoband®

Overview

Anti-PAR1/Thrombin Receptor/F2R Antibody Picoband® is an antibody for F2R detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: F2R (coagulation factor II thrombin receptor); UniProt: P25116; NCBI Gene: 2149
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 47 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-PAR1/Thrombin Receptor/F2R Antibody Picoband® catalog # A03352-1.

Biological background

Biological context: High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

Expression and localization notes: cellular localization: Cell membrane. Multi-pass membrane protein., tissue context: Platelets and vascular endothelial cells..

Common research applications

• Western blotting (WB): Compare F2R levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of F2R in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify F2R-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: Proteinase-activated receptor 1  (PAR1), also known as the coagulation factor II (thrombin) receptor, is a protein that in humans is encoded by the F2R gene. By fluorescence in situ hybridization, this gene is mapped to 5q13, confirming its presence as a single locus in the human genome. PAR1 is a G protein-coupled receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. The expression of PAR1 is both required and sufficient to promote growth and invasion of breast carcinoma cells in a xenograft mouse model. 
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Multi-pass membrane protein.
• Tissue details: Platelets and vascular endothelial cells.
• Research category: Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.