| Field | Specification |
|---|---|
| Target | |
| Alternative names | Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1; polymerase 1, processed C-terminus; Poly [ADP-ribose] polymerase 1, 89-kDa form; ; Poly [ADP-ribose] polymerase 1, processed N-terminus; NT-PARP-1; Poly [ADP-ribose] polymerase 1, 24-kDa form; Poly [ADP-ribose] polymerase 1, 28-kDa form; ]; PARP1; ADPRT; PPOL |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PARP1 recombinant protein (Position: D6-R841). Human PARP1 shares 91.5% and 91.2% amino acid (aa) sequence identity with mouse and rat PARP1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes poly (gene PARP1) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
PARP1 is an abundant nuclear enzyme that detects DNA strand breaks and builds poly(ADP-ribose) chains on itself, histones and repair factors using NAD+. These chains recruit repair proteins to damaged DNA, and PARP1 is the main target of PARP inhibitors used against BRCA-deficient cancers.
| Target | Poly (gene PARP1; UniProt P09874, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PARP1 fragment (Asp6–Arg841), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91.5% and 91.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places poly in the chromosome, nucleus and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
