| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein mono-ADP-ribosyltransferase PARP10; ADP-ribosyltransferase diphtheria toxin-like 10; ARTD10; Poly [ADP-ribose] polymerase 10; PARP-10; PARP10 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PARP10 recombinant protein (Position: E14-E251). Human PARP10 shares 76.5% amino acid (aa) sequence identity with mouse PARP10. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein mono-ADP-ribosyltransferase PARP10 in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 110.0 kDa, and the supplier reports an observed band at about 140 kDa.
PARP10 is a mono-ADP-ribosyltransferase that attaches single ADP-ribose groups to target proteins. It interacts with PCNA and supports DNA damage tolerance during replication, and it has been linked to regulation of GSK3B and NF-κB signaling.
| Target | Protein mono-ADP-ribosyltransferase PARP10 (gene PARP10; UniProt Q53GL7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 110.0 kDa |
| Observed MW | 140 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human PARP10 fragment (Glu14–Glu251), expressed in E. coli. Sequence identity with the mouse ortholog: 76.5%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places protein mono-ADP-ribosyltransferase PARP10 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in spleen and thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
