| Field | Specification |
|---|---|
| Target | |
| Alternative names | Poly [ADP-ribose] polymerase 2; PARP-2; hPARP-2; ADP-ribosyltransferase diphtheria toxin-like 2; ARTD2; DNA ADP-ribosyltransferase PARP2; NAD(+) ADP-ribosyltransferase 2; ADPRT-2; Poly[ADP-ribose] synthase 2; pADPRT-2; Protein poly-ADP-ribosyltransferase PARP2; PARP2; ADPRT2; ADPRTL2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PARP2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18P78 is a rabbit monoclonal antibody that recognizes poly (gene PARP2) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 66.2 kDa, and the supplier reports an observed band at about 66 kDa.
PARP2 is a nuclear poly(ADP-ribose) polymerase closely related to PARP1. It is activated by DNA breaks and adds ADP-ribose chains to proteins to support DNA repair, and together with PARP1 it is inhibited by clinical PARP inhibitors.
| Target | Poly (gene PARP2; UniProt Q9UGN5, human) |
|---|---|
| Clone | 18P78 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 66.2 kDa |
| Observed MW | 66 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:30 |
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PARP2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places poly in the nucleus and chromosome. Tissue expression noted by UniProt (human): Widely expressed, mainly in actively dividing tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
