| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein mono-ADP-ribosyltransferase PARP3; ADP-ribosyltransferase diphtheria toxin-like 3; ARTD3; DNA ADP-ribosyltransferase PARP3; IRT1; NAD(+) ADP-ribosyltransferase 3; ADPRT-3; Poly [ADP-ribose] polymerase 3; PARP-3; hPARP-3; Poly[ADP-ribose] synthase 3; pADPRT-3; PARP3; ADPRT3; ADPRTL3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PARP3 recombinant protein (Position: Q162-Q498). Human PARP3 shares 82.2% amino acid (aa) sequence identity with mouse and rat PARP3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein mono-ADP-ribosyltransferase PARP3 in human, mouse, rat and monkey samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 60.1 kDa, and the supplier reports an observed band at about 60 kDa.
PARP3 is a mono-ADP-ribosyltransferase activated by DNA damage. Unlike PARP1 and PARP2, it attaches single ADP-ribose units, and it supports the repair of DNA double-strand and single-strand breaks.
| Target | Protein mono-ADP-ribosyltransferase PARP3 (gene PARP3; UniProt Q9Y6F1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 60.1 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Monkey, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): monkey COS-7 cells, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue, rat lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Recombinant human PARP3 fragment (Gln162–Gln498), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 82.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and monkey. UniProt places protein mono-ADP-ribosyltransferase PARP3 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
