| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein mono-ADP-ribosyltransferase PARP9; ADP-ribosyltransferase diphtheria toxin-like 9; ARTD9; B aggressive lymphoma protein; Poly [ADP-ribose] polymerase 9; PARP-9; PARP9; BAL; BAL1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PARP9 recombinant protein (Position: R117-E824). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein mono-ADP-ribosyltransferase PARP9 in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 96.3 kDa, and the supplier reports an observed band at about 96 kDa.
PARP9 (also called BAL1) is an interferon-induced member of the PARP family that pairs with the E3 ligase DTX3L. The PARP9–DTX3L complex contributes to DNA damage repair and to interferon-driven antiviral responses.
| Target | Protein mono-ADP-ribosyltransferase PARP9 (gene PARP9; UniProt Q8IXQ6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 96.3 kDa |
| Observed MW | 96 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat skeletal muscle tissue, mouse skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human PARP9 fragment (Arg117–Glu824), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein mono-ADP-ribosyltransferase PARP9 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in lymphocyte-rich tissues, spleen, lymph nodes, peripheral blood lymphocytes and colonic mucosa. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
