| Field | Specification |
|---|---|
| Target | |
| Alternative names | Parvalbumin alpha;PVALB; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Parvalbumin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOFB-16 is a rabbit monoclonal antibody that recognizes parvalbumin alpha (gene PVALB) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 12.1 kDa, and the supplier reports an observed band at about 12 kDa.
Parvalbumin (PVALB) is a small EF-hand protein that binds two Ca2+ ions. It acts as a fast calcium buffer in fast-twitch muscle, helping muscle relax after contraction, and it marks a major class of fast-spiking GABAergic interneurons in the brain.
| Target | Parvalbumin alpha (gene PVALB; UniProt P20472, human) |
|---|---|
| Clone | AOFB-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 12.1 kDa |
| Observed MW | 12 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human cerebellum.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human parvalbumin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Michael P. Zimmerman et al. (2026) Impaired behavioral inhibition in Fmr1 KO mice is linked to disrupted visual cortex theta oscillations. Cell Reports. 10.1016/j.celrep.2026.117590
Sanghamitra Nareddula et al. (2025) FMRP restoration in parvalbumin interneurons: A circuit-specific improvement of visual learning in fragile X syndrome. iScience. 10.1016/j.isci.2025.114132
