| Field | Specification |
|---|---|
| Target | |
| Alternative names | Paired box protein Pax-2;PAX2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Pax2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BDA-16 is a rabbit monoclonal antibody that recognizes paired box protein Pax-2 (gene PAX2) in human and mouse samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 44.7 kDa, and the supplier reports an observed band at about 45 kDa.
PAX2 is a paired-box transcription factor essential for the developing kidney and urogenital tract, eye and midbrain–hindbrain region. It is a common marker of renal epithelium, and PAX2 mutations cause renal coloboma syndrome.
| Target | Paired box protein Pax-2 (gene PAX2; UniProt Q02962, human) |
|---|---|
| Clone | BDA-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.7 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human pax2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places paired box protein Pax-2 in the nucleus. Tissue expression noted by UniProt (human): Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
