| Field | Specification |
|---|---|
| Target | |
| Alternative names | Paired box protein Pax-8; PAX8 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PAX8 recombinant protein (Position: Q137-L450). Human PAX8 shares 95.3% and 95% amino acid (aa) sequence identity with mouse and rat PAX8, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes paired box protein Pax-8 (gene PAX8) in human, mouse and rat samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 48.2 kDa, and the supplier reports an observed band at about 48 kDa.
PAX8 is a paired-box transcription factor required for thyroid development and for expression of thyroid genes such as thyroglobulin and thyroid peroxidase. It is also expressed in kidney and Müllerian tissues and is a common marker in thyroid, renal and ovarian studies.
| Target | Paired box protein Pax-8 (gene PAX8; UniProt Q06710, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 48.2 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SW579 cells, human SK-OV-3 cells, rat kidney tissue, rat NRK cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Recombinant human PAX8 fragment (Gln137–Leu450), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95.3% and 95%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places paired box protein Pax-8 in the nucleus. Tissue expression noted by UniProt (human): Expressed in the excretory system, thyroid gland and Wilms tumors. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
