| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-C; Serine-protein kinase PCK1; PCK1; PEPCK1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PCK1/PEPC recombinant protein (Position: M1-M622). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects phosphoenolpyruvate carboxykinase, cytosolic (gene PCK1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 69.2 kDa, and the supplier reports an observed band at about 69 kDa.
PEPCK-C (PCK1) is the cytosolic phosphoenolpyruvate carboxykinase that converts oxaloacetate to phosphoenolpyruvate. This is a rate-limiting step of gluconeogenesis in liver and kidney, and the enzyme also supports glyceroneogenesis in adipose tissue.
| Target | Phosphoenolpyruvate carboxykinase, cytosolic (gene PCK1; UniProt P35558, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 69.2 kDa |
| Observed MW | 69 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 4 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human PCK1/PEPC fragment (Met1–Met622), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphoenolpyruvate carboxykinase, cytosolic in the cytoplasm and endoplasmic reticulum. Tissue expression noted by UniProt (human): Major sites of expression are liver, kidney and adipocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Weibing Kou et al. (2025) Kanglexin, a new anthraquinone compound, attenuates hepatic fibrosis by regulating the TGF-β/SMADS signaling pathway and glutathione metabolism. International Immunopharmacology. 10.1016/j.intimp.2025.115545
Yuan Yuhao et al. (2025) Study on the expression changes of hepatic Gluconeogenesis-related proteins induced by insulin Overdose-related liver injury. Forensic Toxicology. 10.1007/s11419-025-00754-z
