| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-C; Serine-protein kinase PCK1; PCK1; PEPCK1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PCK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20P18 is a rabbit monoclonal antibody that recognizes phosphoenolpyruvate carboxykinase, cytosolic (gene PCK1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 69.2 kDa, and the supplier reports an observed band at about 69 kDa.
PEPCK-C (PCK1) is the cytosolic phosphoenolpyruvate carboxykinase that converts oxaloacetate to phosphoenolpyruvate. This is a rate-limiting step of gluconeogenesis in liver and kidney, and the enzyme also supports glyceroneogenesis in adipose tissue.
| Target | Phosphoenolpyruvate carboxykinase, cytosolic (gene PCK1; UniProt P35558, human) |
|---|---|
| Clone | 20P18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.2 kDa |
| Observed MW | 69 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human liver cancer tissue, human cervical cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human PCK1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places phosphoenolpyruvate carboxykinase, cytosolic in the cytoplasm and endoplasmic reticulum. Tissue expression noted by UniProt (human): Major sites of expression are liver, kidney and adipocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
