| Field | Specification |
|---|---|
| Target | |
| Alternative names | Choline-phosphate cytidylyltransferase A; CCT-alpha; CTP:phosphocholine cytidylyltransferase A; CCT A; CT A; Phosphorylcholine transferase A; PCYT1A; CTPCT; PCYT1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PCYT1A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21P82 is a rabbit monoclonal antibody that recognizes choline-phosphate cytidylyltransferase A (gene PCYT1A) in human samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 41.7 kDa, and the supplier reports an observed band at about 42 kDa.
CCTα (PCYT1A) is CTP:phosphocholine cytidylyltransferase, the rate-limiting enzyme of the CDP-choline (Kennedy) pathway that makes phosphatidylcholine. It is activated by binding to membranes and is studied in membrane lipid synthesis and lipid droplet biology.
| Target | Choline-phosphate cytidylyltransferase A (gene PCYT1A; UniProt P49585, human) |
|---|---|
| Clone | 21P82 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.7 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human PCYT1A.
Reactivity Notes
The supplier lists reactivity with human. UniProt places choline-phosphate cytidylyltransferase A in the cytoplasm, membrane, endoplasmic reticulum membrane and nucleus. Tissue expression noted by UniProt (human): Brain, placenta, liver, fetal and adult lung.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ende Tao et al. (2025) Study on the molecular mechanism of KPNA2 regulation of myocardial ischemia/delayed reperfusion injury through mitophagy. Cellular Signalling. 10.1016/j.cellsig.2025.112157
