| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death protein 1;Protein PD-1;hPD-1;CD279;PDCD1;PD1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PD1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BEG-16 is a rabbit monoclonal antibody that recognizes programmed cell death protein 1 (gene PDCD1) in human samples and is validated for IHC. The predicted molecular weight is 31.6 kDa, and the supplier reports an observed band at about 32 kDa.
PD-1 (PDCD1) is an inhibitory receptor on activated T cells, B cells and other immune cells. When bound by PD-L1 or PD-L2, it recruits the phosphatase SHP-2 to dampen T-cell receptor signaling, helping maintain self-tolerance; blocking PD-1 is the basis of widely used cancer immunotherapies.
| Target | Programmed cell death protein 1 (gene PDCD1; UniProt Q15116, human) |
|---|---|
| Clone | BEG-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.6 kDa |
| Observed MW | 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunohistochemistry | 1:50–1:200 |
|---|
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PD1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places programmed cell death protein 1 in the cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Meng Yang et al. (2015) T-cell immunoglobulin mucin-3 expression in bladder urothelial carcinoma: Clinicopathologic correlations and association with survival. Journal of Surgical Oncology. 10.1002/jso.24012
Wang YH et al. (2014) Effect of TLR4 and B7-H1 on immune escape of urothelial bladder cancer and its clinical significance. Asian Pacific Journal of Cancer Prevention. 10.7314/apjcp.2014.15.3.1321
