| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; PDCD4; H731 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PDCD4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACAE-16 is a rabbit monoclonal antibody that recognizes programmed cell death protein 4 (gene PDCD4) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 51.7 kDa, and the supplier reports an observed band at about 52 kDa.
PDCD4 (programmed cell death 4) is a translational repressor that binds the RNA helicase eIF4A and blocks its helicase activity and its association with eIF4G, reducing cap-dependent translation initiation. It also dampens JNK/AP-1 signaling, and because it acts as a tumor suppressor that restrains neoplastic transformation and invasion, it is widely studied in cancer biology and microRNA-21 regulation.
| Target | Programmed cell death protein 4 (gene PDCD4; UniProt Q53EL6, human) |
|---|---|
| Clone | ACAE-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.7 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human PDCD4.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places programmed cell death protein 4 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Up-regulated in proliferative cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Junchen et al. (2021) Andrographolide Suppresses the Growth and Metastasis of Luminal-Like Breast Cancer by Inhibiting the NF-κB/miR-21-5p/PDCD4 Signaling Pathway. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2021.643525
