| Field | Specification |
|---|---|
| Target | |
| Alternative names | 2',5'-phosphodiesterase 12; 2'-PDE; 2-PDE; Mitochondrial deadenylase; PDE12 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PDE12 recombinant protein (Position: E36-K607). Human PDE12 shares 89.9% and 91.3% amino acid (aa) sequence identity with mouse and rat PDE12, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 2',5'-phosphodiesterase 12 (gene PDE12) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 67.4 kDa, and the supplier reports an observed band at about 70 kDa.
PDE12 is a mitochondrial 2′,5′-phosphodiesterase that degrades 2′,5′-linked oligoadenylates (2-5A), the second messengers made by interferon-induced OAS enzymes to activate RNase L. By removing 2-5A it acts as a brake on this antiviral pathway, and it is studied in innate immunity and mitochondrial RNA processing.
| Target | 2',5'-phosphodiesterase 12 (gene PDE12; UniProt Q6L8Q7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 67.4 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, human PC-3 cells, human RT4 cells, human U251 cells, human Caco-2 cells, human HepG2 cells, human HeLa cells, human HEL cells, rat brain tissue, rat thymus tissue, rat PC-12 cells, mouse brain tissue, mouse thymus tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human PDE12 fragment (Glu36–Lys607), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 89.9% and 91.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 2',5'-phosphodiesterase 12 in the mitochondrion matrix. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
