| Field | Specification |
|---|---|
| Target | |
| Alternative names | High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A; PDE8A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PDE8A recombinant protein (Position: R32-E495). Human PDE8A shares 80.5% amino acid (aa) sequence identity with mouse PDE8A. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A (gene PDE8A) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 93.3 kDa, and the supplier reports an observed band at about 93 kDa.
PDE8A is a high-affinity, cAMP-specific phosphodiesterase that is insensitive to the broad inhibitor IBMX. It helps set basal cAMP levels, has been linked to steroidogenic and germ cell regulation, and can bind RAF1 to modulate EGF-driven ERK signaling. It is studied in endocrine signaling and T cell biology.
| Target | High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A (gene PDE8A; UniProt O60658, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 93.3 kDa |
| Observed MW | 93 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human RT4 cells, human A549 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): SH-SY5Y cells.
Immunogen
Recombinant human PDE8A fragment (Arg32–Glu495), expressed in E. coli. Sequence identity with the mouse ortholog: 80.5%.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Expressed in most tissues except thymus and peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
