Anti-PDGF B Rabbit Monoclonal Antibody

SKU:BHA21010624
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Overview
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Anti-PDGFB antibody (Rabbit monoclonal, clone 31P34, IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes platelet-derived growth factor subunit B. Observed band in Western blot: about 27 kDa.
Target PDGFB
clone number 31P34
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M00348 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB
      • Application details: WB 1:500-2000
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target PDGFB
Alternative names Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Becaplermin; PDGFB; PDGF2; SIS
UniProt # P01127
Host Rabbit
Clone 31P34
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human PDGF B
Molecular weight 27.3 kDa
Purification Affinity-chromatography
Cellular localization Secreted.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00348
Main SKU BHA21010624
Rabbit Monoclonal

Product Overview

Clone 31P34 is a rabbit monoclonal antibody that recognizes platelet-derived growth factor subunit B (gene PDGFB) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 27.3 kDa, and the supplier reports an observed band at about 27 kDa.

PDGF-B is a growth factor that forms PDGF-BB homodimers or PDGF-AB heterodimers and signals through PDGF receptors. It is a strong mitogen for fibroblasts and smooth muscle cells, recruits pericytes to new blood vessels and contributes to wound healing.

Target Platelet-derived growth factor subunit B (gene PDGFB; UniProt P01127, human)
Clone 31P34
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 27.3 kDa
Observed MW 27 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Immunogen

Synthetic peptide based on the sequence of human PDGF B.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt describes platelet-derived growth factor subunit B as a secreted protein. Tissue expression noted by UniProt (human): Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 27.3 kDa; observed band at about 27 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human PDGF B.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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