| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet-derived growth factor subunit B;PDGF subunit B;PDGF-2;Platelet-derived growth factor B chain;Platelet-derived growth factor beta polypeptide;Proto-oncogene c-Sis;Becaplermin;PDGFB;PDGF2, SIS; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PDGF beta recombinant protein (Position: S82-T190). Human PDGF beta shares 89% amino acid (aa) sequence identity with both mouse and rat PDGF beta. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects platelet-derived growth factor subunit B (gene PDGFB) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 27.3 kDa, and the supplier reports an observed band at about 13 kDa.
PDGF-B is a growth factor that forms PDGF-BB homodimers or PDGF-AB heterodimers and signals through PDGF receptors. It is a strong mitogen for fibroblasts and smooth muscle cells, recruits pericytes to new blood vessels and contributes to wound healing.
| Target | Platelet-derived growth factor subunit B (gene PDGFB; UniProt P01127, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 27.3 kDa |
| Observed MW | 13 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat cardiac muscle tissue, rat brain tissue, mouse cardiac muscle tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human PDGF beta fragment (Ser82–Thr190), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 89%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes platelet-derived growth factor subunit B as a secreted protein. Tissue expression noted by UniProt (human): Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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