| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet-derived growth factor receptor alpha;PDGF-R-alpha;PDGFR-alpha;2.7.10.1;Alpha platelet-derived growth factor receptor;Alpha-type platelet-derived growth factor receptor;CD140 antigen-like family member A;CD140a antigen;Platelet-derived growth factor alpha receptor;Platelet-derived growth factor receptor 2;PDGFR-2;CD140a;PDGFRA;PDGFR2, RHEPDGFRA; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PDGFRA, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes platelet-derived growth factor receptor alpha (gene PDGFRA) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 122.7 kDa, and the supplier reports an observed band at about 123 kDa.
PDGFRα is a receptor tyrosine kinase that binds PDGF-A, PDGF-B and PDGF-C dimers and signals through PI3K, PLCγ and SHP2. It drives proliferation, migration and differentiation of mesenchymal cells and is required for skeletal, gut and neural crest development. Activating PDGFRA mutations occur in some gastrointestinal stromal tumors.
| Target | Platelet-derived growth factor receptor alpha (gene PDGFRA; UniProt P16234, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 122.7 kDa |
| Observed MW | 123 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HT1080 cells, human Caco-2 cells, human U2OS cells, human PC-3 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, human breast cancer tissue, human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human PDGFRA. The peptide sequence is conserved in mouse. It differs from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places platelet-derived growth factor receptor alpha in the cell membrane, cell projection and Golgi apparatus. Tissue expression noted by UniProt (human): Detected in platelets (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Barbara L. Wehde et al. (2018) Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression. Cell Reports. 10.1016/j.celrep.2018.10.063
Soliman Soha A et al. (2022) Role of Uterine Telocytes During Pregnancy. Microscopy and Microanalysis. 10.1093/micmic/ozac001
Yun-Mei Sun et al. (2017) PDGFRα Regulated by miR-34a and FoxO1 Promotes Adipogenesis in Porcine Intramuscular Preadipocytes through Erk Signaling Pathway. International Journal of Molecular Sciences. 10.3390/ijms18112424
