| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD140b; PDGFRB; PDGFR; PDGFR1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human PDGFRB recombinant protein (Position: E911-L992). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes platelet-derived growth factor receptor beta (gene PDGFRB) in human, mouse and rat samples and is validated for ELISA, flow cytometry and Western blot. The predicted molecular weight is 124.0 kDa, and the supplier reports an observed band at about 190 kDa.
PDGFRβ is a receptor tyrosine kinase activated by PDGF-BB, PDGF-AB and PDGF-DD. Its signaling recruits pericytes and vascular smooth muscle cells to growing blood vessels and drives mesenchymal cell proliferation and migration, so it is a common pericyte marker and is studied in vascular development, fibrosis and cancer.
| Target | Platelet-derived growth factor receptor beta (gene PDGFRB; UniProt P09619, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 124.0 kDa |
| Observed MW | 190 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SH-SY5Y cells, human U2OS cells, rat ovary tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): SH-SY5Y cells.
Immunogen
Recombinant human PDGFRB fragment (Glu911–Leu992), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places platelet-derived growth factor receptor beta in the cell membrane, cytoplasmic vesicle and lysosome lumen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chen Yang et al. (2025) Electroacupuncture Prevents Against AD‐Like Phenotypes in APP/PS1 Mice: Investigation of the Mechanisms From Cerebral Microangiopathy. CNS Neuroscience & Therapeutics. 10.1002/cns.70696
Si-Wen Chen et al. (2008) RNA interference targeting the platelet-derived growth factor receptor β subunit ameliorates experimental hepatic fibrosis in rats. Liver International. 10.1111/j.1478-3231.2008.01759.x
J-X Wang et al. (2017) GLI2 induces PDGFRB expression and modulates cancer stem cell properties of gastric cancer. European Review for Medical and Pharmacological Sciences. PMID: 28975979
Gao Qian et al. (2025) Pan-cancer analysis of PDGFRB: Laying the foundation for the development of targeted immunotherapy drugs. Medicine. 10.1097/MD.0000000000041797
J.-H. Xiao et al. (2006) Effects of isoliensinine on angiotensin II-induced proliferation of porcine coronary arterial smooth muscle cells. Journal of Asian Natural Products Research. 10.1080/1028602042000325609
Ya-Juan Zhao et al. (2012) Protective effects of glutamine in a rat model of endotoxemia. Molecular Medicine Reports. 10.3892/mmr.2012.1007