| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial;1.2.4.1;PDHE1-A type I;PDHA1;PHE1A; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PDHA1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AODI-16 is a rabbit monoclonal antibody that recognizes pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (gene PDHA1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 43.3 kDa, and the supplier reports an observed band at about 43 kDa.
PDHA1 is the alpha subunit of E1, the first catalytic component of the mitochondrial pyruvate dehydrogenase complex. With PDHB it uses thiamine pyrophosphate to decarboxylate pyruvate, the step that commits glycolytic carbon to acetyl-CoA and the TCA cycle. Its activity is switched off by PDK phosphorylation, and PDHA1 deficiency is a well-known cause of lactic acidosis.
| Target | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (gene PDHA1; UniProt P08559, human) |
|---|---|
| Clone | AODI-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.3 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human PDHA1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
