| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein disulfide-isomerase A2;5.3.4.1;Pancreas-specific protein disulfide isomerase;PDIp;PDIA2;PDIP; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PDI |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOBI-16 is a rabbit monoclonal antibody that recognizes protein disulfide-isomerase A2 (gene PDIA2) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 58.2 kDa, and the supplier reports an observed band at about 58 kDa.
Protein disulfide-isomerase A2 (PDIA2, also called PDIp) is a member of the PDI family that is expressed mainly in the pancreas. Besides chaperone activity that limits aggregation of misfolded proteins, it can bind estrogens inside cells and may help regulate their local levels, and it is studied in pancreatic protein folding.
| Target | Protein disulfide-isomerase A2 (gene PDIA2; UniProt Q13087, human) |
|---|---|
| Clone | AOBI-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.2 kDa |
| Observed MW | 58 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PDI.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein disulfide-isomerase A2 in the endoplasmic reticulum lumen. Tissue expression noted by UniProt (human): Highly expressed in pancreas (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
