| Field | Specification |
|---|---|
| Alternative Names | Protein disulfide-isomerase A6;5.3.4.1;Endoplasmic reticulum protein 5;ER protein 5;ERp5;Protein disulfide isomerase P5;Thioredoxin domain-containing protein 7;PDIA6;ERP5, P5, TXNDC7; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human PDIA6 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PDIA6 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AOBE-16; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-PDIA6/Erp5 Rabbit Monoclonal Antibody catalog # M03813. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PDIA6 (Protein disulfide-isomerase A6).
- Antibody format: Monoclonal; clone AOBE-16; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PDIA6 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. . Reported cellular localization context: Endoplasmic reticulum lumen . Cell membrane . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). . Tissue expression notes (as provided): Expressed in platelets (at protein level). .
Research relevance and current trends
- Research context keywords from the source record include: ER Proteins,Protein Trafficking,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate PDIA6 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PDIA6 expression by Western blot in cell or tissue lysates, Localize PDIA6 by immunofluorescence/immunocytochemistry in cultured cells, Quantify PDIA6-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 43 kDa; calculated MW: 48121 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 43 kDa
- Cellular localization (provided): Endoplasmic reticulum lumen . Cell membrane . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). .
- Tissue details (provided): Expressed in platelets (at protein level). .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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