| Field | Specification |
|---|---|
| Target | |
| Alternative names | [Pyruvate dehydrogenase; acetyl-transferring; ] kinase isozyme 1, mitochondrial; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1; PDK1; PDHK1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PDK1 Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACOF-16 is a rabbit monoclonal antibody that recognizes [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (gene PDK1) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 49.2 kDa, and the supplier reports an observed band at about 46 kDa.
Pyruvate dehydrogenase kinase 1 (PDK1) is a mitochondrial kinase that phosphorylates the PDHA subunit and switches off the pyruvate dehydrogenase complex, reducing conversion of pyruvate to acetyl-CoA. It is a direct HIF-1 target that shifts cells toward glycolysis under hypoxia, and it is studied in cancer metabolism. It should not be confused with PDPK1, the AGC kinase also called PDK1.
| Target | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (gene PDK1; UniProt Q15118, human) |
|---|---|
| Clone | ACOF-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 49.2 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PDK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Expressed predominantly in the heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Pengzhen Wang et al. (2019) Icariin increases chondrocyte vitality by promoting hypoxia-inducible factor-1α expression and anaerobic glycolysis. Knee. 10.1016/j.knee.2019.09.012
