| Field | Specification |
|---|---|
| Target | |
| Alternative names | [Pyruvate dehydrogenase; acetyl-transferring; ] kinase isozyme 3, mitochondrial; Pyruvate dehydrogenase kinase isoform 3; PDK3; PDHK3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PDK3 recombinant protein (Position: Y84-Q406). Human PDK3 shares 97.2% amino acid (aa) sequence identity with mouse PDK3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial (gene PDK3) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 46.9 kDa, and the supplier reports an observed band at about 47 kDa.
Pyruvate dehydrogenase kinase 3 (PDK3) is a mitochondrial kinase that inhibits the pyruvate dehydrogenase complex through phosphorylation of PDHA1. It reduces glucose oxidation in favor of fat metabolism, for example during fasting, and like other PDK isoforms it is studied in metabolic regulation and cancer metabolism.
| Target | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial (gene PDK3; UniProt Q15120, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 46.9 kDa |
| Observed MW | 47 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, human 293T cells, human RT4 cells, human SiHa cells, human Jurkat cells, human U251 cells, human A549 cells, rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human meningioma tissue, human rectum adenocarcinoma tissue, mouse testis tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): U251 cells.
Immunogen
Recombinant human PDK3 fragment (Tyr84–Gln406), expressed in E. coli. Sequence identity with the mouse ortholog: 97.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Expressed in heart, skeletal muscle, spinal cord, as well as fetal and adult brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
