| Field | Specification |
|---|---|
| Target | |
| Alternative names | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial;2.7.11.2;Pyruvate dehydrogenase kinase isoform 4;PDK4;PDHK4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PDK4, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial (gene PDK4) in human and rat samples and is validated for Western blot. The predicted molecular weight is 46.5 kDa, and the supplier reports an observed band at about 46 kDa.
Pyruvate dehydrogenase kinase 4 (PDK4) is a mitochondrial kinase that shuts down the pyruvate dehydrogenase complex by phosphorylating its E1α subunit. Its expression rises strongly in fasting, diabetes and high-fat feeding, conserving glucose by shifting muscle and heart toward fatty acid oxidation, so it is a common marker in studies of metabolic flexibility.
| Target | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial (gene PDK4; UniProt Q16654, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 46.5 kDa |
| Observed MW | 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, human A549 cells, rat skeletal muscle tissue, rat heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human PDK4. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kolnes Kristoffer J. et al. (2025) Effects of seven days’ fasting on physical performance and metabolic adaptation during exercise in humans. Nature Communications. 10.1038/s41467-024-55418-0
Wei Wei et al. (2023) METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression. Journal of Experimental & Clinical Cancer Research. 10.1186/s13046-023-02732-y
Mohib Mohammad Mohabbulla et al. (2024) Eplerenone, a mineralocorticoid receptor inhibitor, reduces cirrhosis associated changes of hepatocyte glucose and lipid metabolism. Cell Communication and Signaling. 10.1186/s12964-024-01991-2
Gudiksen Anders et al. (2017) Effects of training status on PDH regulation in human skeletal muscle during exercise. Pflugers Archiv-European Journal of Physiology. 10.1007/s00424-017-2019-6
