| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial; PDPr; KIAA1990 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PDPR recombinant protein (Position: E155-E655). Human PDPR shares 94.4% amino acid (aa) sequence identity with mouse PDPR. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial (gene PDPR) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 99.4 kDa, and the supplier reports an observed band at about 99 kDa.
PDPR is the regulatory subunit of mitochondrial pyruvate dehydrogenase phosphatase. It lowers the sensitivity of the catalytic subunit PDP1 to Mg2+, an inhibition that the polyamine spermine can relieve, and so it helps fine-tune reactivation of the pyruvate dehydrogenase complex.
| Target | Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial (gene PDPR; UniProt Q8NCN5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 99.4 kDa |
| Observed MW | 99 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human PDPR fragment (Glu155–Glu655), expressed in E. coli. Sequence identity with the mouse ortholog: 94.4%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial in the mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
